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The pellet was resuspended in 2 ml ammonium chloride/Tris-chloride (pH 7.2) (erythrocyte lysing buffer), incubated at room temperature for 5 min, then supplemented with 1 ml FCS and centrifuged subsequently at 1433 r.p.m

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This later contributed to growing interest in oral capsule administration rather than injection-only protocols

Traumatic brain injury-related emergency department visits, hospitalizations, and deaths - United States, 2007 and 2013