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The oxygen consumption rate (OCR) was measured using the XFe96 extracellular flux analyzer (Agilent Technologies, Inc.) as previously described (32)

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9.5.3 Rapalogs and calorie restriction mimetics Rapamycin (sirolimus), a macrolide compound derived from Streptomyces hygroscopicus, is a potent inhibitor of the mechanistic target of rapamycin (mTOR) complex