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doi: 10.1016/j.cellsig.2007.05.013 32 KopczyskaEMakarewiczR
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The frozen cells expressing GST-IP6K1 were suspended in the lysis buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 1 mM MgCl 2 , 5 U/mL benzonase, cOmplete (Roche), EDTA-free Protease Inhibitor Cocktail), and centrifuged at 15,000 g for 30 min
[DOI] [PMC free article] [PubMed] [Google Scholar] Henrquez, G
Its essential to avoid situations that use up lots of energy (like fasting) as that can trigger the condition
