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The mitochondria produces other forms of energy through the citric acid cycle (TCA) in the form of NADH and FADH

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Therefore, 25l of each selected serum sample was albumin-depleted using a Montage Albumin Deplete kit (Millipore, Billerica, MA) and diluted in sample buffer containing 7M urea, 2M thiourea, 1% w/v SB 3-10, 3% w/v CHAPS, 0.25% v/v Bio-Lyte 3/10 ampholytes (Bio-Rad Laboratories Inc., Hercules, CA), and 1.5% v/v protease inhibitor cocktail (Sigma, St