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Calculate Volume : Find the appropriate volume of sterile water based on desired level

Proteomics 108, 3054

doi: 10.1136/gut.2007.136366

The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates

Kamaraj, B., Rajendran, V., Sethumadhavan, R., Kumar, C