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A general clinical examination, determination of the level of 25-(OH)-D, ultrasound densitometry (QUS), molecular diagnostics (BSML polymorphism of the VDR gene) were performed

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MOTS-c, as a mitochondrial-derived peptide, offers a unique approach to enhancing cellular energy, improving insulin sensitivity, and counteracting age-related metabolic decline by optimizing foundational cellular processes, particularly through AMPK activation and mitochondrial function

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The sections were treated with goat anti-Rat Alexa Fluor 488 IgG (H+L) (1:500, A-11006, Invitrogen) and DAPI (5 mg ml 1 ) for visualization