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Proc Natl Acad Sci USA 96, 46864691 (1999)

The cells were centrifuged at 1,000 rpm for 11 min, and the cells were resuspended in 500 l PI and incubated at 37 C in the dark for 35 min

In CKD, uremic toxins (e.g., indoxyl sulfate) synergize with iron overload to suppress GPX4, further enhancing ROS-mediated eryptosis ( 4 The role of eryptosis in patients 4.1 The role of eryptosis in non-dialysis-dependent patients Eryptosis, the programmed death of erythrocytes characterized by PS exposure on the cell membrane, has emerged as a critical contributor to renal anemia in non-dialysis-dependent chronic kidney disease (NDD-CKD), with estimated glomerular filtration rate (eGFR) identified as its key determinant ( r 2 = 0.22) and the NDD-CKD subgroup ( r 2 = 0.10)

Nature 543 , 637646 (2017)