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To elute the peptides, 100 L of elution buffer (60% acetonitrile and 0.1% TFA) was applied to the columns and centrifuged at 800 rpm for 3 min

Among the tested analogs, compounds with 4-methoxy ( 10a ), 4-hydroxy ( 10d ) or 2-hydroxy ( 10e ) substitutions show higher potent inhibition, suggest that changes to the basic resveratrol structure by a nitrogen atom resulted an increase in tyrosinase inhibitory effects

Misuse can lead to serious risks, including copper toxicity or adverse effects like angiogenesis, which may be dangerous in cases of cancer