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Koyama laboratory, and were maintained in Roswell Park Memorial Institute 1640 medium (RPMI) (Thermo Scientific, #31800105) containing 10 % FBS, 1 % P/S, and 50 mol L 1 -mercaptoethanol

These technologies demonstrate significant application value in cancer mechanism research, immunotherapy evaluation, target discovery, and new drug development (Sanchez-Carbayo, 2006

J Biol Chem 264:1351923 Chen J, Stewart V, Spyrou G, Hilberg F, Wagner EF, Alt FW (1994) Generation of normal T and B lymphocytes by c-jun deficient embryonic stem cells

Haggerty et al

coli using the co-expression approach used for RiPP biosynthetic pathway elucidation