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Glycyl-L-histidyl-L-lysinecopper (GHK-Cu), an endogenous tripeptidemetal complex, has attracted increasing interest due to its multifaceted biological roles in tissue repair, anti-inflammatory regulation, extracellular matrix remodeling, and redox homeostasis

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Relative to other affinity interactions, the binding between GST and glutathione is pretty slow, so youll want to incubate your protein of interest with the beads for around 30 minutes to an hour before uncapping the column and letting the flow-through drip out the bottom