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Korean Journal of Anesthesiology

The isolated cDNAs from the pool enriched in the fourth and fifth rounds were ligated into the pGEM-T-Easy vector (Promega), using DNA Ligation Kit Mighty Mix (Takara Bio), and cloned into DH5 competent cells

Resurfacing is a strategy for reducing the immunogenicity of non-human antibodies by replacing solvent-accessible framework residues with their human equivalents

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