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Subsequently, the reporter gene, CHK2 plasmid and renilla luciferase plasmid was co-transfected into HEK293-CHK2-KO cells for 48 h under normoxia or hypoxia
Raghad Haddad, Email: [email protected]
doi: 10.1249/01.Mss.0000155432.67020.88 181 GleesonMMcDonaldWAPyneDBCrippsAWFrancisJLFrickerPAet al
H 2 O 2 is slightly a ROS as well as the simplest form of peroxide
The knockdown efficiency was confirmed by more than 65% of mRNA reduction compared to the control group in cells using real time PCR (see Supplementary Table 1)
