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Consistency is more important than expensive procedures

synthesized 10 polyphenylated FMP compounds and evaluated their performance as MALDI-MS reactive matrices for analyzing small molecules with phenolic hydroxyl, primary amine, and secondary amine groups

We also tested whether the toxic load of BA in the absence of LROs might impair the induction of the HSR, by employing a similar, 4-h BA preconditioning as in the thermotolerance assay, followed by a 2-h heat stress

& Kajiyama, H

As reduced GSH is the first line of defense against ROS, its concentration rapidly decreases by oxidant treatment 14 , which may, at least partially, explain the anti-aging effect of mild ROS