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Centrifuge at 10,000 rpm to collect the supernatant, then combine it with the previously collected supernatant in a 10 mL centrifuge tube for volumetric measurements
The regulation of HIFs is just one example of how ROS and cellular ratios of various TCA cycle metabolites, i.e., mitochondrial metabolism, can control gene expression and cellular responses (39)
Figure 1C)
doi: 10.1007/s11259-023-10265-0
Mulkens et al
