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(4/17/18) mp3 #264 Dean Haspiel Cartoonist and playwright Dean Haspiel joins the show to talk about his new play, The Last Bar At The End Of The World (running April 10-15, 2018!) and how he looks at his life & career after turning 50

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For protein expression, the transformed cells were grown in MGYH medium (1.34% YNB, 1% glycerol, 4 10 5 % biotin, 0.004% histidine) to an OD 600 = 3.06.0 and then induced in MMH medium (1.34% YNB, 0.5% methanol, 4 10 5 % biotin, 0.004% histidine) at 30 C for an additional 48 h

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