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Bioinformatics analysis Hierarchical clustering analyzes were performed using the GenePattern software ( Raw RNA-Seq reads were pre-processed with Trimmomatic software ( Homo sapiens reference transcriptome (Gencode, release 36) and the total number of reads mapped per transcript and the number of transcripts per million were calculated with Salmon software ( summarizeToGene function of the R Tximeta package ( P value less than 0.05 obtained in the False Discovery Rate (FDR) analysis were considered differentially expressed (DEG) and submitted to functional enrichment analysis with Ingenuity Pathways Analysis software (IPA, Qiagen) using default parameters

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The procedure itself was smooth, with minimal discomfort