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Finally, GHK has been shown to be able to serve as a cell adhesion molecule, which means that it helps cells to attach themselves to the extracellular matrix
Next, the sections were incubated with a secondary antibody (Alexa Fluor 594-conjugated goat anti-mouse IgM, #A21044, 1:100 dilution, Thermo Fisher Scientific) at 37C for 60 min
Based on these findings, we maintained the same sequential pain testing protocol as used in above chemogenetic inhibition for subsequent neural manipulation experiments
