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The frequency of administration depends on your individual needs and will be determined by your doctor based on your medical condition

February 2003 study A 2003 mouse study found that TB4 in a solution or gel form promoted accelerated wound healing in healthy, diabetic, and aged mice

doi:10.1080/0092623X.2015.1033579

If you do not have any of the conditions below then it is likely that your low vitamin B12 is due to your diet, in which case it is not a medical issue and can be corrected with oral supplements you can buy over the counter or online

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