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After washing to remove unbound primary antibody, they were incubated with a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (ZSGB-Bio, China) for GPX4 or goat anti-mouse antibody (ZSGB-Bio, China) for PTGS2 at 37 C for 1 h

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It is found in nearly every cell in the body, with higher concentrations in tissues such as the: Liver Lungs Brain Skin Eyes Gastrointestinal tract Glutathione plays a role in cellular processes related to antioxidant activity and maintaining internal balance