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One major advantage of using recombinant antibody (rAb)-based ELISA is to create rAbs-reporter fusions through genetic engineering, preventing the need for chemical production of antibody-reporter compounds or dependence on commercially available secondary antibodies [132,133]
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[DOI] [PMC free article] [PubMed] [Google Scholar] 31.Gulyaeva N.V
Fluorescence was measured using a Varioskan Flash multimode reader (ThermoFisher Scientific) at excitation/emission 495/515 nm, with 150 L per sample in black 96-well plates
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