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In dermal fibroblasts cultured from aged donors, GHK-Cu treatment (2 M) increases TIMP-1 mRNA expression by approximately 25%, contributing to reduced net collagenolytic activity and preservation of existing matrix structures

Future studies should expand the sample size, comprehensively collect clinical indicators, identify pathogenic variants, and apply the Mendelian randomization (MR) method to predict causal relationships, with experimental verification as the final step

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A scoring system was used to grade the degree of lung injury in lung tissue analysis (Gojkovic et al., 2021a

In the future, the RPL intervention paradigm may shift from single-pathway repair toward more comprehensive strategies centered on system reprogramming, multi-target coordination, and dynamic feedback regulation, thereby providing stronger technical support and mechanistic foundations for individualized RPL management
