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Amino Acids The contents of aspartic acid (Asp), glutamic acid (Glu), serine (Ser), glycine (Gly), lysine (Lys), histidine (His), threonine (Thr), arginine (Arg), alanine (Ala), proline (Pro), cysteine (Cys), tyrosine (Tyr), valine (Val), methionine (Met), isoleucine (Ile), leucine (Leu) y phenylalanine (Phe) were determined from 1 g of BB by high-performance liquid chromatography (HPLC), using the AccQ-Tag Kit (Waters Chromatography, S.A., Barcelona, Espaa, following the manufacturers instructions

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