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Acknowledgements We are grateful for the support from the Laboratory Animal Center at National Taiwan University, College of Medicine and Flow Cytometric Analyzing and Sorting Core of the First Core Laboratory, National Taiwan University College of Medicine for providing the service
Sequencing libraries were prepared from the isolated gDNA using two rounds of PCR amplification with Herculase II DNA polymerase (Agilent)

Gls deletion promotes energy expenditure and metabolic health As a hallmark feature of beige adipocytes is lipid droplet multilocularity 29 , we next investigated the cellular composition of iWAT using single-nucleus RNA sequencing
