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The successful insertion of the resistance cassette in mutant strains selected on chloramphenicol/HBA agar was confirmed by polymerase chain reaction (PCR) analysis of genomic DNA from selected mutants

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Hepatology 42(5):9871000 Bugianesi E, Gastaldelli A, Vanni E, Gambino R, Cassader M, Baldi S, Ponti V, Pagano G, Ferrannini E, Rizzetto M (2005) Insulin resistance in non-diabetic patients with non-alcoholic fatty liver disease: sites and mechanisms

Column chromatography is a pivotal technique in the purification of polysaccharides, with ion exchange chromatography and gel filtration chromatography being the most frequently employed methods