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Membranes were washed for 3 5 min with 0.1% Tween 20 (TBS-Tween) followed by a 2 h incubation at room temperature with the appropriate secondary antibody: anti-mouse IgG HRP-linked (Cell Signaling, #7076) or anti-rabbit IgG HRP-linked (Cell Signaling, #7074) diluted 1:1000 in EveryBlot Blocking Buffer

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