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Red blood cells were lysed with 500 l of 1 BD FACS lysing solution (BD, 349202) for 5 min at room temperature, stopped with 3 ml of PBS, and stained with the antibody mixture consisting of Mac1, Gr1, B220, and CD3 for 15 min at 4 C, followed by flow cytometry analysis (BD, Fortessa)

[DOI] [PubMed] [Google Scholar] 131.Aldini G., Altomare A., Baron G., Vistoli G., Carini M., Borsani L., Sergio F

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The biphasic response suggests possible mechanisms: lower doses (nanogram range) might work through growth factor upregulation and signaling cascade amplification, while higher doses (microgram range) might involve direct receptor activation or substrate provision for cellular repair

& Rudel, T