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Then, cells were treated according to instructions in a dark environment for 3060 min

Store in a cool, dry place, protected from direct sunlight

GSH in fact is both a nucleophile and a reductant, and therefore can react with electrophilic or oxidizing species rendering the former molecules more soluble and excretable, and the latter unable to interact with more critical cellular constituents such as lipids, nucleic acids and proteins

Quantitative determinations for manganese and calcium were obtained by the external standard calibration with four standards (0, 1, 5, 10 g L 1 ) prepared daily in the same buffer used for sample preparation, at the same dilution and HNO 3 concentration

1 Introduction Diabetes mellitus (DM) is a group of endocrine and metabolic disorders characterized by hyperglycemia, and its incidence has been steadily increasing ( Figure 1 )