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Reverse transcription of total mRNA were performed at 1 g of total mRNA in 25 l final volume using random primers and avian myeloblastosis virus reverse transcriptase

Irwin, D

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However, the p K a of the cysteine sulfhydryl side-chain is 8.4, and therefore, at intracellular pH ranges (7.07.4), these groups exist mostly in the non-nucleophilic thiol state