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Blood pressure effects
Unsurprisingly, the GHSR-1a receptor has been shown to downregulate in response to various stimuli, including ghrelin- and ghrelin-ligand mediated activation [177,178,179]
Hamrita, B
Finally, the 2 Ct method was used to determine relative expression, with the housekeeping gene Glyceraldehyde 3-phosphate dehydrogenase ( GAPDH ) acting as the internal reference [34]