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As an auxiliary method to HPLC, it improves the reliability of analytical results

However, long-term safety in humans requires further research

Quantitative measurement of selected metabolites in cultured cells The relative levels of selected metabolites (NA, SAM, SAH, NAD + ) regulated by cellular energy expenditure pathways associated with NNMT were simultaneously detected using LC/MS/MS and MRM ratios

All experiments were carried out in accordance with the Guide for the Care and Use of Laboratory Animals [20] and with approval from the Institutional Animal Care and Use Committee at the University of Texas Medical Branch

Effect of NNMT inhibitor on adipocyte differentiation quantitated with oil red O staining 3T3-L1 cells were cultured in 60 mm diameter dishes (8.410 4 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 M dexamethasone, 10 g/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)