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Description
I had a similar issue with the first shot and its happening again after the 2nd

Subsequently, cells were wounded by pipette tips and washed twice with media to remove detached cells, and photomicrographs of initial wounds were taken using Canon PowerShot A640 camera (at 100 magnification)

When the slave enters operational mode, it sends PDO messages periodically based on configured communication parameters or event triggers
It helps lay down the initial plumbing, so to speak

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