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Then antigen retrieval was performed with 1X Target Retrieval Solution, pH 6.0 (Dako) at 125 C for 4 min in a Retriever 2100 (Aptum Bio), followed with blocking using 10% goat serum in PBS for 60 min at RT

In addition, if possible, you can use more mineral spray

Spleens from MSK1/2 (serine/threonine kinase)-deficient mice accumulate eryptotic erythrocytes, and their erythrocytes are characterized by enhanced eryptosis following glucose depletion or hyperosmotic shock [152]

The following sgRNAs for GGT1 CRISPRi were used: sgGGT1 1 (HL-ID: 3) forward: 5-CACCGGTGCCTCCCACTGTCCGCCG-3