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These wide-ranging effects explain why glutathione depletion has been associated with accelerated aging, increased disease susceptibility, impaired detoxification capacity, and compromised immune responses

Protein concentration was determined using a BCA protein assay kit (Beyotime Bio, P0010)

Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling

Glutathione Assay Kit (Fluorometric) (ab65322) provides a simple in vitro assay for detection of total glutathione changes during cellular response to toxicity, apoptosis and other conditions

As it turns out, the connection is significant