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EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections
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Stain-free gels were then wet-transferred onto polyvinylidene difluoride (PVDF, Millipore) membranes at 300 mV constantly for 1 h, and equal transfer was routinely confirmed by Ponceau S staining
