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For this purpose, PCR primers were designed as follows, where a polyhistidine tag (His-Tag) was incorporated in the reverse primer: VEGF-F: 5-ACATCAGGTACCATGAACTTTCTGCTGTCTTGG -3, and VEGF-R: 5-ACATCTGGTACCAGCGGCCGCATGATGATGATGATGATGCCGCCTCGG CTTGTCAC -3

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doi: 10.1016/0024-3205(82)90677-4

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Absolute gene expression was determined by calculating the percentage of gene expression relative to human -actin using the Eq

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