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Next, cDNA was synthesized by reverse transcription using the QuantiTect Reverse Transcription Kit from Qiagen according to the manufacturers instructions, and qRT-PCR was finally performed using a QuantiTect SYBR Green PCR Kit and QuantiTect Primer Assays (Qiagen) on a LightCycler 480 instrument (Roche)

Morris G, Walker AJ, Walder K, Berk M, Marx W, Carvalho AF, et al

Identifying and targeting cancer-specific metabolism with network-based drug target prediction

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