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A homogenization buffer was prepared, composed of 300 mM saccharose, 10 mM K-HEPES, and 1 mM EGTA, which were diluted in ultrapure water

But remember, doing this will make your solution runny

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Conversely, deacetylation of G6PD mediated by deacetylase Sirt2 enhances the enzymatic activity of G6PD and counteracts excessive oxidative stress (Wang et al., 2014