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Moreover, the amide group from glutamine can also be transferred to formylglycinamide ribonucleotide (FGAR), which subsequently forms phosphoribosyl-formylglycinamidine (FGAM) through the catalytic activity of 5'-phosphoribosylformylglycinamidine synthase (PFAS) during nucleotide synthesis
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U-2OS and KHOS-24OS cells were cultured in 90% RPMI-1640 containing 10% FBS
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