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For IHC assays of tissue sections, the tumor sections were prepared the same as above and Ki-67 staining was performed using a universal IHC kits (PV-9000, ZSGB-Bio Co., Ltd, China) according to the manufacturer's instructions

Lab monitoring: Ask your doctor for comprehensive metabolic panel at 6 months

Morimoto R

Additional Tips: Justify your choice: Clearly document the rationale for selecting the animal model in your study protocol