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Description
For intracellular staining of phospho(p)-mTOR (Fisher Scientific 15549836) and p-S6 (eBioscience 15528216), cells were first stained for extracellular markers, fixed in 2% FA at RT, and stained for p-mTOR and p-S6 diluted 1:200 in saponin 5 g/mL
Handb Environ Chem
doi:10.1016/j.resmic.2018.09.002 Cozzolino M, Vitagliano A, Pellegrini L, et al
Most peptides are broken down by digestive enzymes before they can be absorbed, which is why most are injected
One shortcoming is the evolution of the perfusion protocol alongside our learning curve
