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In vivo and vitro experiments revealed that hypoxic stress enhances yes-associated protein (YAP) binding to HIF-1, activating GLUT1 transcription thus promotes glycolysis in PTC [177]

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Afterwards cells were stained by Immunofluorescence with rabbit anti-SARS-CoV-2 nucleocapsid antibody (GeneTex, Cat# GTX135357, RRID:AB_2868464) 1:1,000 in PBS and with goat anti-rabbit AlexaFluor 594 antibody (Thermo Fisher Scientific, Cat# A-11012, RRID:AB_2534079) 1:2,000 in PBS

Consistent with the modest hypocotyl shortening, thermo-induced expression of the PIF4 targets IAA19 and IAA29 trended lower in the mTAD lines, but only the reduction in IAA19 level within the higher-PIF4 line pair ( PIF4/pif4 #17 and PIF4 mTAD /pif4 #13) reached statistical significance, whereas changes in the lower-PIF4 pair ( PIF4/pif4 #15 and PIF4 mTAD /pif4 #10) and those in IAA29 expression across both pairs were not significant (Fig

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