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Targeted analysis of APF To develop an MRM method, the highest single charged (1482.8 m / z ) and double charged (741.88 m / z ) states of the APF peptide were used as precursor ions, and the five product ions (539.32 m / z , 620.38 m / z , 626.39 m / z , 638.39 m / z , 826.5 m / z ) were used as transitions to monitor the APF peptide