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Tracking these metrics every 2-4 weeks provides more meaningful progress assessment than daily scale weight, which fluctuates with hydration, bowel contents, and other transient factors

To eliminate formazan crystals, 200 Lof DMSO was then replaced with the media, and absorbance values were measured at 570 nm, using a spectrophotometric Plate Reader (ELx808, Lonza Biotech Co., Switzerland)