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Silencing PFKFB4 increases F2,6-BP, an allosteric activator of PFK1, diverting glucose flux towards glycolysis

To quantify the mScarlet fluorescence in individual chromatography fractions, 100 L samples were each transferred to a 96-well Nunc black microwell plate (Thermo Fisher Scientific) and the fluorescence intensities were measured in a Synergy 2 microtiter plate reader with 530/25 nm and 590/35 nm filters for excitation and emission, respectively

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2025 Oct 31;17(11):1415

However, this particular dose is indicated for sleep-related pathologies such as jet-lag and works by targeting the central nervous system where canonical melatonin receptors are abundantly expressed (69)