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The clinician will conduct several evaluations that include developmental and medical history, behavioral observations, and standardized tests like the ADOS-2 (Autism Diagnostic Observation Schedule, 2nd Edition), says Delventhal

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The membranes were blocked with 5% bovine serum albumin (BSA) or 5% dry skimmed milk in Tris-buffered saline containing 0.05% (v/v) Tween-20 (TBST) for 2 h and incubated with the appropriate primary antibody in blocking buffer for 2 h at room temperature