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it encompasses motility, morphology, DNA integrity, and the hormonal environment that supports sperm production

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Incorporating 1% Triton X-100 or 2% n-octylglucoside can significantly improve the elution efficiency of certain GST-tagged proteins

Within the carbohydrate metabolism pathways, these metabolites were primarily enriched in pathways related to pyruvate metabolism, butyrate metabolism, glycolysis/gluconeogenesis, the tricarboxylic acid (TCA) cycle, and oxalate and dicarboxylate metabolism, all of which are associated with SCFA metabolism