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4.6.4 Possible side effects and potential health risks Despite its benefits, RJ may cause allergic reactions, including skin rashes and pruritus, particularly in sensitive individuals ( 4.7 Nutrients are currently recognized as beneficial for dry eye disease Nutrient therapy is a significant component in the holistic management of DED, supplementing medical treatments

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This was achieved through upregulation of protein levels and subsequent increased activity of glutamate-cysteine ligase (GCL), the rate-limiting enzyme in glutathione synthesis (Jarrett et al., 2008)

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Moreover, from the present findings, it was found that creatine or LC exhibits potent anti-oxidant and anti-inflammatory activity via regulation of several varieties of free radicals and cytokines by improving the effect of anti-oxidant enzymes such as CAT, SOD, GSH, etc., and inflammatory cytokines such as TNF, ILs, NF-Kb, etc