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9 When patients present relatively late after ingestion, oral N-acetylcysteine protocols provide a higher dose given over a longer period, which may be an advantage

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Pulsatile stimulation reduces that danger, facilitating improved long-term regulation [1][6]

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Spheroplasts were then washed three times with YPD/1 M sorbitol and once with 1 M sorbitol, and lysed as described previously (Waters and Blobel, 1986) with a douncer in lysis buffer [20 mM HEPES pH 7.5, 100 mM KOAc, 2 mM Mg(OAc) 2 , 10% Glycerol, 1 mM DTT, 0.5 mM PMSF, and complete EDTA-free protease inhibitors (Cytiva)]