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Adherent cells were scrapped, then centrifuged at 4C and 13000 g for 15 min, and the resulting supernatants processed using established protocols.[19] Intracellular levels of 1-MNA and as well as the IS were determined from LC/MS/MS peak areas

It functions as an AR agonist and has been extensively investigated in studies of receptor binding dynamics, ligand-receptor interactions, transcriptional regulation, and AR-mediated signaling mechanisms