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After 48 h, HC11 mammary epithelium cells (ATCC, CRL-3062) were infected, and stable integrants were selected with puromycin (2 g/mL)
First, these modifications increase the hydrophobicity and decrease the aqueous solubility of bile acids, resulting in a marked lowering of the monomeric concentration of bile acids in aqueous solution
Samples were homogenized using a Mini-BeadBeater-8 (BioSpec) for three rounds of 20 s each, with 30 s resting on ice between each round
10.1016/0092-8674(91)90397-H 3
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